Prevalence of Campylobacter Jejuniin Poultry Products and Beef in Owerri Municipality
Ohanusi, I. N.
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The prevalence of Campylobacter species in beef and poultry products sold in Owerri, Nigeria, was assessed. Exactly 400 samples were analysed and isolates were recovered on selective media and characterized using the API Campy Kits. Antimicrobial activity against the isolates was carried out by dehydrogenase assay method using the alcoholic extracts of utazi, scent leaf, and commercial antibiotics. Prevalence data revealed that Campylobacter jejuni ssp jejuni 2 was predominant in all samples with a prevalence of 53(13.25 %), 51(12.75 %), 51(12.75 %) and 49(12.25 %) respectively for the Ready-to-eat meat, Turkey meat, Chicken meat and Beef. Campylobacter jejuni sspjejuni1 had a prevalence of 20% on the Ready-to-eat meat, 17% each on the others. Campylobacter coli had a prevalence of 22 %, 27 %, 28 % and 34 % on the Ready-to-eat meat, Turkey meat, Chicken meat and Beef respectively while the least abundant isolate was the Campylobacter jejuni bv faecaliswith an occurrence of 5(1.25%), 5(1.25%), 4(1 %) and 0(0 %) respectively. Species of Campylobacter have demonstrated varied susceptibility patterns. Result showed that isolates were susceptible to treatment with gentamycin, amikacin and ciprofloxacin compared to the activity of other tested antibiotics on the isolates. Ciprofloxacin demonstrated the best activity on the isolates. All isolates demonstrated multiple resistances to ceftriaxone, cefotaxime, ceftazidime, amoxicillin, tetracycline and cephalexin. Gamma plots of the inhibition of dehydrogenase activity by ethanolic and methanolic extracts of utazi and scent leaves produced IC10 values of 835.19 g/ml and 817.76 g/ml; as well as 542.51 g/ml and 1100.64 g/ml respectively. This provides quality evidence that spices if used on foods can inhibit the activity of Campylobacter sp. However, the use of antibiotics in the cultivation of farm animals should be discouraged as it contributes immensely to the antibiotic resistance reported.